The Ss. Stimmate di San Francesco ("Church of the Holy Stigmata of St. Francis")is a church in central Rome, Italy, in the Rione Pigna, sited where previously there was a church called Ss. Quaranta Martiri de Calcarario.
A first church in the place was consecrated in 1297. In 1597 the land was given by Pope Clement VIII to the Confraternita delle Ss.Stimmate; the construction a new building was completed in 1714, on designs by Giovanni Battista Contini.
The facade was designed by Antonio Canevari, in a plan recalling Pietro da Cortona's style. In the center niche is a statue of the saint receiving the stigmata as he looks heavenward. The ceiling has a Glory of St. Francis by Luigi Garzi, while the first chapel to the right has a Flagellation by Marco Benefial with a cupola frescoed by Giovanni Odazzi. The main altarpiece is a Saint Francis with stigmata (1719) by Francesco Trevisani. In the first chapel the left is a Ss. Quaranta Martiri (Holy 40 martyrs) by Giacinto Brandi, author of another St. Francis with Stigmata in the Hall of Stimmate.